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991.
国产五种菊属植物的核型研究 总被引:21,自引:0,他引:21
本文对国产5种菊属植物的核型进行了研究,结果为:D. indicum 2n=2x=18=16m 2st,2n=4x=36=28m 6sm 2st;D. lavandulifolium 2n=2x=18=14m 4sm,2n=4x=36=28m 6sm 2st;D. lavandulifolium var.seticuspe 2n=2x=18=12m 6sm;D. chanetii 2n=4x=36=20m 14sm 2st,2n=6x=54=38m 14sm 2st;D. potentilloides2n=2x=18=14m 2sm 2st;D. vestitum 2n=6x=54=38m 16sm.核型分析的结果表明,本文所研究类群中出现的多倍体多为异源多倍体;根据对核型资料、形态特征及地理分布特点的综合分析,作者认为多倍化是菊属野生种进行的主要途径。 相似文献
992.
利用秆维管束进行中国散生竹类的聚类分析 总被引:2,自引:0,他引:2
本文是应用模糊聚类分析方法研究中国散生竹类分类的一次尝试。分类特征采用了竹秆上、中、下三段各类型维管束数,方法上使用了模糊(Fuzzy)直接聚类分析进行综合分析。经电子计算机运算后,不仅取得了与传统分类基本一致的分类结果,同时也表明这种方法较之其它一些植物数量分类方法简便易行,此外还讨论了一些中国散生竹类分类上的问题。 相似文献
993.
994.
茶园冬季乔木落叶的分解和矿质元素释放 总被引:1,自引:0,他引:1
在我国南方存在着一种传统植茶方式——茶林复合生态系统,近年来人们已逐步认识到它在维持土壤肥力,抗御自然灾害和保证茶叶内质特性等方面的作用,然而对冬季乔木落叶分解和矿质元素释放的作用尚无报道。本文是对安徽省黄山市休宁县茶树-乌桕复合园和茶树-板栗复合园的冬季乔木落叶分解的研究,为全面认识茶林复合生态系统的性质提供依据。 相似文献
995.
Takeshi Shimomura Toshiyuki Honda Chiharu Oouchi Jun Kondo Kazuhiro Nagaike 《Cytotechnology》1991,6(1):1-11
The recombinant human apolipoprotein E (Apo-E) produced by Chinese hamster ovary cells (CHO-322 cells) in serum free culture was degraded to 24K and 23K fragments that contained N-terminal amino acid. The degradation site of Apo-E to 24K fragment was between Arg180 and Leu181 and the C-terminal amino acid of 23K fragment was Gly169. In fetal bovine serum (FBS)-containing culture, the degradation was inhibited. However, in calf serum (CS) the inhibitory activity was not detected. Thus, we attempted the purification of the factor with this inhibitory activity from FBS. A protease inhibitor was purified to give a single peak from FBS by ammonium sulfate precipitation and combination of several column chromatographies. When this FBS-derived protease inhibitor (FBS-d-PI) was added to serum-free culture of CHO-322 cells, degradation of recombinant Apo-E to the 24K and 23K fragments was dose-dependently suppressed and accumulation of intact Apo-E in culture supernatant was observed. FBS-d-PI was found to be a glycoprotein with relative molecular size of 75K daltons under reducing condition, and 85K daltons under nonreducing condition by SDS-PAGE. A complex of FBS-d-PI and a cellular protease was also detected in culture supernatant by western blot analysis using mouse monoclonal antibodies against FBS-d-PI. 相似文献
996.
Method for the isolation of high-quality RNA from grape berry tissues without contaminating tannins or carbohydrates 总被引:21,自引:1,他引:20
Grape berries contain compounds that aggregate with and precipitate RNA in the presence of chaotropic agents or phenol. The
procedure described here extracts RNA from finely ground tissues using mild denaturants, and selectively precipitates the
aggregate-forming material with 30% ethanol. The resulting RNA is suitable for northern blot analysis and translationin vitro. 相似文献
997.
Protein variation in wild Atlantic salmon, with particular reference to southern Ireland 总被引:1,自引:0,他引:1
The extent of genetic variation in wild Atlantic salmon parr, Sulmo salur L., from river systems in Ireland, Iceland and eastern Canada, was investigated using starch gel electrophoresis. Within Ireland, seven polymorphic enzyme loci ( sAAT-4 *, GPI-1 *, IDDH-1 *, IDDH-2 *, IDHP-3 *, MDH-3 * and mMEP-2 *) were screened in nine different rivers and nine tributaries from the River Blackwater. Significant heterogeneity in gene frequencies occurred between riverine samples and between samples from tributaries of the River Blackwater. Variation between tributaries was as great as between rivers elsewhere in the country. Levels of population differentiation were comparable to those found in other regions throughout the range of the species, and temporal stability in gene frequencies was apparent when the results were compared with previously published data. Screening of riverine samples from Iceland and eastern Canada (Newfoundland and New Brunswick) allowed the Irish results to be considered in a broader context. Irish salmon cluster in the western European group, to which may be added Icelandic populations. Salmon from eastern Canada show a high level of genetic distinctiveness from the European group. 相似文献
998.
Mitsuo Satoh Shinji Hosoi Seiji Sato 《In vitro cellular & developmental biology. Plant》1990,26(11):1101-1104
Summary The protease activity in serum-free conditioned medium of chinese hamster ovary (CHO) cells was measured using peptidyl (or
aminoacyl)-4-methylcoumaryl-7-amides (MCAs) as the substrates. Aminopeptidase increased in level as amounts of nonviable cells
increased during cultivation in serum-free medium, indicating that the activity seems to be originated from intracellular
proteases. The activity toward Boc-Leu-Arg-Arg-MCA, which was strongly inhibited by p-chloromercuribenzonate and N-ethylmaleimide,
was the strongest among those toward peptidyl-MCAs in the conditioned medium within 48 h-cultivation in serum-free medium.
In contrast to the case of aminopeptidase activity, the endopeptidase activity decreased in level after 48 h-cultivation although
amounts of nonviable cells increases. Thus, CHO cells continuously secrete the cysteine proteases.
This work was supported by the management of the Research Association for Biotechnology as a part of the R&D of Basic Technology
for Future Industries sponsored by NEDO (New Energy and Industrial Technology Development Organization). 相似文献
999.
中华猕猴桃胚乳植株后代的观察 总被引:2,自引:0,他引:2
对438株定植的中华猕猴桃胚乳培.养的试管苗,经四年的田间观察,并进行连续二年结果分析。与对照的母株相比,胚乳植株在株形、叶片大小、果实形态及果实的主要营养成分含量上都有较大的变化。同时还发现,由同一块愈伤组织诱导的胚乳试管苗后代中也有雌、雄性别的分化。胚乳植株后代的多样性,可为中华猕猴桃的选种及品种繁育提供丰富的材料。 相似文献
1000.
黄连木五倍子的生态研究与利用 总被引:1,自引:0,他引:1
一、产地自然概况黄连木五倍子最早发现于河南省南部信阳市郊贤山脚下(东经114°0′,,北纬32°10′)。该地为低山丘陵,海拔90一320m,南部紧连鸡公山厂傍依南湾水库和狮河。 相似文献